[Histonet] ZYMED Histostain Plus Kit on animal tissue

Kim Merriam kmerriam2003 <@t> yahoo.com
Wed May 14 12:51:11 CDT 2008

I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue.  It might be worth giving Zymed a call to find out about this kit.
 Kim Merriam, MA, HT(ASCP)
Cambridge, MA

----- Original Message ----
From: Mary L Giebel/FS/VCU <mlgiebel <@t> vcu.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, May 14, 2008 12:56:51 PM
Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue

At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. 
Thank you for your help.
Mary Giebel

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