Sending privately Re: [Histonet] Glucose oxidase method for blocking endogenous peroxidase

[Gayle Callis]

Nadia,

I will attach the method and all the regeant needs to you privately and 
where you can purchase the correct glucose.  No, glucose D-(=) Glucose will 
NOT work.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715
----- Original Message ----- 
From: "Gale, Nadia" <Ngale <@t> bccancer.bc.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, May 14, 2008 11:05 AM
Subject: [Histonet] Glucose oxidase method for blocking endogenous 
peroxidase


I've searched the archives and cannot find what I am after... I'd like
to try the glucose oxidase method for blocking endogenous peroxidase in
tissue sections for IHC.

What is a suitable replacement for the B-d(+)glucose G5250 discontinued
by Sigma?  Will the D-(+)-Glucose G8270 work?  This is 4% beta isomer
and 96% alpha.


Thanks for your help,
Nadia

Nadia Gale
Lead Histotechnologist
Centre for Translational and Applied Genomics (CTAG)
3427-600 West 10th Avenue
Vancouver, BC V5Z 4E6
tel 604-877-6000 ext 2426
fax 604-877-6089
ngale <@t> bccancer.bc.ca

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet