[Histonet] Glucose oxidase method for blocking endogenous peroxidase

Gale, Nadia Ngale <@t> bccancer.bc.ca
Wed May 14 12:05:39 CDT 2008


I've searched the archives and cannot find what I am after... I'd like
to try the glucose oxidase method for blocking endogenous peroxidase in
tissue sections for IHC.
 
What is a suitable replacement for the B-d(+)glucose G5250 discontinued
by Sigma?  Will the D-(+)-Glucose G8270 work?  This is 4% beta isomer
and 96% alpha.
 
 
Thanks for your help,
Nadia
 
Nadia Gale
Lead Histotechnologist
Centre for Translational and Applied Genomics (CTAG)
3427-600 West 10th Avenue
Vancouver, BC V5Z 4E6
tel 604-877-6000 ext 2426
fax 604-877-6089
ngale <@t> bccancer.bc.ca
 


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