I am curious how labs determine the drying time and temps on their IHC slides prior to retrieval and IHC staining? Overnight at RT, 1 hour at 60 C, 30 min at 70 C? I have seen all these in use but no actual documentation of why? Jeanne Clark [40.gif] 233 McCarty Hollow Road Telford, Tn. 37690 423-612-1213