[Histonet] Hematoxylin Shortage??

Bryan Llewellyn llewllew <@t> shaw.ca
Mon Mar 31 17:39:26 CDT 2008

I always thought that hematoxylin, coming as it does from a tree, was 

I went to Bromley Technical College on a Friday evening to be taught by 
Wallington, 1963 and 1964 possibly, the brain fails sometimes.  I ran afoul 
of him on one occasion, although I really didn't understand why at the time. 
He had told us the Schiff reagent is a leucobase thing and I had read 
somewhere else that it wasn't, so I asked in all innocence.  I wished I 
hadn't.  After not so oblique references to being careful that I didn't fail 
my exams (he was an examiner for the IMLT in histology Finals) I kept my 
mouth shut.  As it happens, he was an examiner for me at my practical and my 
oral and he passed me as well.

His great innovation was proving secondary fixation actually worked, as I 
recall.  I also recall he had no hesitation debunking the old mythological 
procedures.  Everyone has thei foibles, me included I'm sure.

Bryan Llewellyn

----- Original Message ----- 
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, March 31, 2008 12:45 PM
Subject: RE: [Histonet] Hematoxylin Shortage??

> Kemlo: I was attempting to be flippant: you know, we go to the Supermarket 
> and buy "organic" produce.....
> I do not deride such people as Wallington: they paved the way. Massive 
> respect.
> Well, I have more respect for Katie Page, who actually tried to make thing 
> WORK, rather than regurgitating methodology...( even if she found her last 
> lover in Eric......
> according to the Papers I read..no way do I state this as my own )
> Just that many got stuck in a rut.
> We do.....
> As I am approaching his age, when he dismissed me, I feel a similar 
> "rear-guard" action.
> However, he had moved on by challenging those that came before him, also, 
> surely?
> Not sure but, Wallington served me very well, in the early days of my 
> passion for histology.
> Respect
> ( hur, hur, I still feel that way about another "bible" Theory and 
> Practise of histological Techniques"...however, seems to me that the 
> majority of Chapters are just regurgitations.....gimme Kiernan any time 
> ;-)
> Back tomounting media: Sure, each  mounting medium has it's 
> place.....particularly regarding the all-important RI.
> However, I have never noticed the difference, in routine Histological 
> situations.
> The navel-gazing regarding RI was based upon historical microscopic 
> inadequacies...
> Which are still evident today, sigh.....not many set up their microscopes 
> ( Kohler-wise) to avoid that GLARE.......sigh.
> It is extremely important that new generations understand Kohler 
> illumination, Firstly. Mounting medium, secondarily!
> So many times, I see brightfield images that have that "glare"...
> Yes, Barry: Sincere respects, but, "I feel canada balsam is 
> better"......is it or not?
> I think I understand your stance, as I come from the Canada balsam skool.
> Luverly smell, too....;-)
> However....open for discussion.
> When I worked in dental stuff, we always used CB: when I became in charge, 
> I swapped to DPX: good, good.
> Dried quicker, gave a result quicker......who gave a damn about the 
> minutiae of RI.
> Anyway, Barry, ground sections do not comply.....they are too thick!
> I know, I did them for several years: also , I did methyl methacrylate 
> bone stuff, mounting in DPX....;-)
> C
> Best wishes,
> Carlos.
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