[Histonet] Hematoxylin Shortage??
carl.hobbs <@t> kcl.ac.uk
Mon Mar 31 14:45:32 CDT 2008
Kemlo: I was attempting to be flippant: you know, we go to the Supermarket
and buy "organic" produce.....
I do not deride such people as Wallington: they paved the way. Massive
Well, I have more respect for Katie Page, who actually tried to make thing
WORK, rather than regurgitating methodology...( even if she found her last
lover in Eric......
according to the Papers I read..no way do I state this as my own )
Just that many got stuck in a rut.
As I am approaching his age, when he dismissed me, I feel a similar
However, he had moved on by challenging those that came before him, also,
Not sure but, Wallington served me very well, in the early days of my
passion for histology.
( hur, hur, I still feel that way about another "bible" Theory and Practise
of histological Techniques"...however, seems to me that the majority of
Chapters are just regurgitations.....gimme Kiernan any time ;-)
Back tomounting media: Sure, each mounting medium has it's
place.....particularly regarding the all-important RI.
However, I have never noticed the difference, in routine Histological
The navel-gazing regarding RI was based upon historical microscopic
Which are still evident today, sigh.....not many set up their microscopes
( Kohler-wise) to avoid that GLARE.......sigh.
It is extremely important that new generations understand Kohler
illumination, Firstly. Mounting medium, secondarily!
So many times, I see brightfield images that have that "glare"...
Yes, Barry: Sincere respects, but, "I feel canada balsam is better"......is
it or not?
I think I understand your stance, as I come from the Canada balsam skool.
Luverly smell, too....;-)
However....open for discussion.
When I worked in dental stuff, we always used CB: when I became in charge, I
swapped to DPX: good, good.
Dried quicker, gave a result quicker......who gave a damn about the minutiae
Anyway, Barry, ground sections do not comply.....they are too thick!
I know, I did them for several years: also , I did methyl methacrylate bone
stuff, mounting in DPX....;-)
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