[Histonet] embedding problem small zebrafish

[Geoff McAuliffe]

Greetings Barbara:

    How are you fixing and processing the fish? We do plastic embedding 
of z-fish embryos and have no problems with morphology.

Geoff

Barbara Verstraeten wrote:
> Hello,
>
> I work on zebrafish from 44 hours to 6 days old (so they are very 
> small; max. 0.5 cm).
> I would like to perform immostainings on them (such as E-cadherin 
> staining). I've embedded my species in technovit 9100 but the cutting 
> of such small animals is very difficult.
> I did some stainings already and the antibody works but the problem is 
> that my tissue is of very bad quality!! When I have 50 sections only 1 
> is usefull.
> This is not very good!
>
> Can someone give me any suggestions on how to embed them so that they 
> are more easy to section and that I can still stain them with 
> different antibodies?
>
> Thank you all in advance!
>
> Kind regards,
>
> Barbara Verstraeten
> VMDB
> University of Ghent
> Belgium
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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