[Histonet] embedding problem small zebrafish
Geoff McAuliffe
mcauliff <@t> umdnj.edu
Mon Mar 31 14:11:06 CDT 2008
Greetings Barbara:
How are you fixing and processing the fish? We do plastic embedding
of z-fish embryos and have no problems with morphology.
Geoff
Barbara Verstraeten wrote:
> Hello,
>
> I work on zebrafish from 44 hours to 6 days old (so they are very
> small; max. 0.5 cm).
> I would like to perform immostainings on them (such as E-cadherin
> staining). I've embedded my species in technovit 9100 but the cutting
> of such small animals is very difficult.
> I did some stainings already and the antibody works but the problem is
> that my tissue is of very bad quality!! When I have 50 sections only 1
> is usefull.
> This is not very good!
>
> Can someone give me any suggestions on how to embed them so that they
> are more easy to section and that I can still stain them with
> different antibodies?
>
> Thank you all in advance!
>
> Kind regards,
>
> Barbara Verstraeten
> VMDB
> University of Ghent
> Belgium
>
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>
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
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