[Histonet] embedding problem small zebrafish

Barbara Verstraeten barbara.verstraeten <@t> ugent.be
Tue Mar 25 03:50:27 CDT 2008


I work on zebrafish from 44 hours to 6 days old (so they are very  
small; max. 0.5 cm).
I would like to perform immostainings on them (such as E-cadherin  
staining). I've embedded my species in technovit 9100 but the cutting  
of such small animals is very difficult.
I did some stainings already and the antibody works but the problem is  
that my tissue is of very bad quality!! When I have 50 sections only 1  
is usefull.
This is not very good!

Can someone give me any suggestions on how to embed them so that they  
are more easy to section and that I can still stain them with  
different antibodies?

Thank you all in advance!

Kind regards,

Barbara Verstraeten
University of Ghent

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