[Histonet] Decal procedures-assessing endpoints

[peter.craven <@t> nhs.net]

Greg 
We use a chemical end point test where we neutralise 5ml of the used decal
solution with ammonia then add an equal quantity of saturated Ammonium
Oxalate and leave for 30 mins if the solution goes cloudy we change the
solution the specimen is in and keep decalcifying. If it stays clear we
process and cut the tissue. The tissue need to be in the decalcifier for at
least overnight to give an accurate reading, works with acid decalcifying
solutions but not with EDTA. We use a litmus paper strip to identify the
acid has been neutralised.

Hope this helps 

Peter L Craven FIBMS
BMS2
Pathology Department
Raigmore Hospital
Inverness
IV2 3UJ
Tel 01463 704000 ext 4269


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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Greg Dobbin
Sent: 27 March 2008 14:40
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal procedures-assessing endpoints

Hello Colleagues,
Can some of you share with me, your guidelines for determining
appropriate submersion times for sections of bone or other calcified
bits in RDO (Trade name) post-fixation but prior to processing
(obviously).  I guess it is a  subjective call by necessity!?   
Thanks,
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

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