[Histonet] RNase free condition in OCT sectioning
hfedor <@t> jhmi.edu
Wed Mar 26 10:13:06 CDT 2008
This is how I usually go about it.
Use 100% ethanol or RNase ZAP to wipe down the cryostat handle, glass door, buttons, everything that may be touched. Then clean all tools, Brush, forceps etc. with ethanol. Place paper towels on top of the cryostat, and write out the slides with gloves on my hands on top of the paper towels. If you are going to be sectioning and collecting into tubes, put the tubes onto crushed dry ice into a small container, inside the cryostat, to keep the sections well frozen and cut down on static build-up.
Use a new, clean blade (wiped with ethanol) for each sample to keep from contaminating your samples during sectioning.
We have excellent results for LCM and for tube methods.
>>> "FU,DONGTAO" <fudo <@t> ufl.edu> 3/26/2008 10:44 AM >>>
A PI want to get some sections from OCT blocks under RNase free
condition, so they can isolate RNA from them. I will use RNaseZap
to clean work station. What else can I do to prevent RNase
contamination? Any suggestions will be helpful.
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