[Histonet] collagen coating

[koellingr <@t> comcast.net]

depending on how good your inverted TC scope is, when I was doing a lot of tissue culture and preping some plates with collagen, you could see the coverage without stains because you can see the collagen fibers coating the well. Just have to make the light "contrasty" in the plane of view and you can see them.  
Ray Koelling
Phenopath Labs
Seattle, WA

 -------------- Original message ----------------------
From: "Hazel Stevens" <hstevens3 <@t> mail.gatech.edu>
>  
> 
> Hi All,
> 
>  
> 
> I have received excellent responses from Histonet in the past so thought I
> would try this out on you all. 
> 
>  
> 
> I am coating tissue culture plates with collagen and trying to demonstrate
> complete coverage. Other than an antibody to collagen I , is there a simple
> stain I could apply that would demonstrate coverage without dissolving the
> collagen. Collagen seems to dissolve in methanol which seems to be a
> component of some of the basic protein stains. Should I fix before staining?
> 
> 
> Thanks for your help. Much appreciated.
> 
> Hazel 
> 
>  
> 
> Hazel Stevens, 
> 
> Georgia Tech,
> 
> Atlanta, Georgia 30332-0405 U.S.A.
> 
>  
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet