AW: [Histonet] MLH-1,MSH-2,MSH-6

Andi Kappeler kappeler <@t>
Wed Mar 26 05:22:03 CDT 2008

Hi Martha

- MLH-1, clone G168-15, BD Pharmingen, CatNo 550838; HIER-Citrate pH 6.0
(1:50, H1(30).
- MSH-2, clone FE11, Calbiochem (used to be Oncogene Research), CatNo NA27;
HIER-Urea pH 9.5 (1:50, H2(20)).
- MSH-6, clone 44, BD Transduction, CatNo 610919; HIER-Citrate pH 6.0
(1:50, H1(30)).
- PMS-2, clone A16-4, BD Pharmingen, CatNo 556415; HIER-Citrate pH 6.0
(1:100, H1(30)).
Figures in brackets refer to dilutions and retrieval we used when testing
these antibodies on a Bond (with reasonable results, however optimization
still required). Hope this helps. Good luck with testing!

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland 

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Martha
Gesendet: Dienstag, 25. März 2008 18:27
An: histonet <@t>
Betreff: [Histonet] MLH-1,MSH-2,MSH-6

Hello!  I have a request for these antibodies.  We are using the Bond immuno
stainer.  Does anyone have any vendor preferences they can share with me?
Thanks in advance for your help.
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist
Medical Center Medical Center Blvd.
Winston-Salem, NC 27157
mward <@t>
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