[Histonet] RE: Histonet Digest, Vol 52, Issue 9

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Mar 7 07:22:47 CST 2008

The problem with fatty tissue is the fat, not the water. Fatty tissues require more clearing (fat dissolvents) and benefit also from prolonged processing protocols to allow the paraffin to infiltrate the areas where the fat has been removed.
  René J

Ramona Turner <mona_diane <@t> hotmail.com> wrote:
I also would like some advice on processing fatty tissue. I have tried using Pen-Fix prior to processing, but my pathologist complain about an artifact and refuse to use it anymore. Can't explain that one. I am currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing x2. I thought the dehydration step should be increased on the fatty tissue, but you are saying that is not the problem? Can someone else please offer some experience on this issue. 

Ramona Turner, HT (ASCP)
Potomac Hospital
Woodbridge, VA 
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