[Histonet] CD31 vs. Von Willebrand Factor

[Bernice Frederick]

We use an anti-mouse cd31 for mouse tissue from what was Fitzgerald
industries and I believe are now part of Invitrogen. For human I agree with
you Andrea, we use the Dako CD31 for human, same with the VWF. It's
polyclonal - don't know if we've used it on mouse. It does cross react with
pig.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Hooper
Sent: Wednesday, March 05, 2008 5:13 PM
To: jstaruk
Cc: 'histonet'
Subject: Re: [Histonet] CD31 vs. Von Willebrand Factor

Are you staining mouse tissue or human tissue? If mouse tissue, the vWF
antibody from DAKO as well as the goat polyclonal anti-VE-Cadherin Ab from
R&D work very well. If human tissue, the anti-CD31 from DAKO, clone JC/70 is
excellent. 



----- Original Message -----
From: jstaruk <jstaruk <@t> masshistology.com>
Date: Wednesday, March 5, 2008 2:05 pm
Subject: [Histonet] CD31 vs. Von Willebrand Factor

> Hello all,
> 
> I have a request for a CD31 looking for angiogenesis.  I know this 
> is a very
> problematic antibody when working with paraffin sections.  Can the Van
> Willebrand antibody be substituted to obtain the same results?
> 
> Thank you
> 
> Jim
> 
> _____________________
>     Jim Staruk
> Mass Histology Service
> www.masshistology.com
> 
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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> 


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