[Histonet] Paraformaldehyde fixation and membrane Antibody

[karen Cai]

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Hello All,
I am just wondering whether it=92s OK to use 4% paraformaldehyde as
fixation agent for cells when I try to detect the membrane protein.
Which kind of fixation agent is the best? I know formaldehyde is a
cell-permeable agent. So even I don=92t use the Triton X-100 to
permeablize the cell, the reagent can still move readily into living
cells and cause wrong staining. Am I right?
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Thanks in advance,
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Best Regards,
Karen

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Checked by AVG Free Edition.=20
Version: 7.5.516 / Virus Database: 269.21.4/1313 - Release Date:
3/5/2008 9:50 AM
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