[Histonet] Tris buffer

[Min-Han Tan]

Dear Margaret,

Analyzing the recipe you have provided : you are preparing a solution of pH
7.1, Tris-HCl 50 mM, with NaCl concentration of 150 mM.

This is based on the Henderson Hasselbach equation = 8.3 + log (1.4/121)/
(6/157.6), where 8.3 is the pKa of Tris. The molarity of Tris is based on
standard molecular weight calculations, Tris FW = 121 and Tris-HCl FW =
157.6.

This seems like a reasonable recipe (NaCl = 150 mM - physiologic), except
that perhaps I might aim for 7.4 or 7.2 from sheer familiarity. :)

Regards,

/min-han


Min-Han Tan
MBBS, MRCP(UK)
Department of Medical Oncology
National Cancer Centre Singapore



On Wed, Mar 5, 2008 at 5:46 AM, Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
wrote:

> We need to update our SOP's and I can't find a reference for our tris
> buffer recipe.  I can find many recipes but not ours.  Our recipe is as
> follows:
>
>
>
> 6 grams Trizma Hydrochloride
>
> 1.4 grams Trizma base
>
> 8.75 grams Sodium chloride
>
> Qs with water to 1 liter
>
>
>
> Do any of you have a reference for this recipe?
>
>
>
> Margaret Perry HT (ASCP)
>
> IHC Lab Manager Veterinary Science
>
> Animal Disease Research and Diagnostic Lab
>
> South Dakota State University
>
> Box 2175 North Campus Drive
>
> Brookings SD 57007
>
>
>
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>



-- 
/min-han