[Histonet] Tris buffer

Perry, Margaret Margaret.Perry <@t> sdstate.edu
Tue Mar 4 15:46:15 CST 2008

We need to update our SOP's and I can't find a reference for our tris
buffer recipe.  I can find many recipes but not ours.  Our recipe is as


6 grams Trizma Hydrochloride

1.4 grams Trizma base

8.75 grams Sodium chloride

Qs with water to 1 liter


Do any of you have a reference for this recipe?  


Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007


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