[Histonet] Liquid Nitrogen guns

Ingles Claire CIngles <@t> uwhealth.org
Tue Mar 4 13:21:48 CST 2008

Hey gang:
Sorry it has taken me so long to get back to you with the info. It's been crazy here for the past couple weeks. Anyway, here goes. We order our Liquid Nitrogen guns from Brymill. They are out of Ellington CT 06029. Their number is 800-777-CRYO. They are the guns that are also usually used in Dermatology clinics. We also have a couple of 2 gallon Dewars flasks to hold the LN after we dispense it from the bigger 50L tank. I'm not sure where the large cryo tanks or Dewars flasks can be gotten. I believe there are actually smaller 25 L cryo tanks as well. We get the actual LN from a medical gas company (Airgas). The gun works essentially like the cryocool cans, but freezing of specific areas is much easier. The fatty areas can be targeted and the more fiberous/muscle areas and OCT/TBS can be avoided so they are not over cooled and become friable. We regularly cut whole 2x2 cm pieces of pure fat with great results. It takes a bit longer, but there is actually tissue on the slide instead of a hole or just a blob of fat.
I'll try and help with any questions anyone has.(I'll try to respond to you faster next time too).
Claire Ingles
UW Hospital and Clinics
Madison WI

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