AW: [Histonet] Washing out formalin fixation

Gudrun Lang gu.lang <@t>
Tue Mar 4 10:04:30 CST 2008

Formaldehyde is bound to makromolecules as methylol-groups or Schiff-bases
in the first step. These formations are said to be unstable and can be
removed. The crosslinking is a second step, where stable methylenbridges are
built. These should be stable and withstand the washing.
The linkages between formaldehyd and various tissue-compounds have different
power, depending on the reactionpartners and the milieu. 

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Johnson,
Gesendet: Montag, 03. März 2008 20:33
An: 'histonet <@t>'
Betreff: [Histonet] Washing out formalin fixation

Last week, a researcher here asked me what the chemical mechanism was of
washing out the effects of formalin fixation on the tissues with running
water. In other words, how does it work? Anybody here know?

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

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