[Histonet] HMB45 antibody

MaryAnn Dixon DixonM <@t> vetmed.ufl.edu
Thu Jun 26 12:56:05 CDT 2008

Hi histonetters,


Anyone out there successfully using a monoclonal HMB45 antibody on
canine tissues?  I think I've read a dozen papers all with different
protocols and my eyes are about to pop out of my head!  Currently I'm at
a 1:50 dilution with a pretreatment in a citrate buffer (pressure
cooker). I have my choice of a polymer based detection as well as a
biotinylated detection.  Any help would be appreciated.  Thanks in


MaryAnn Dixon  BS

Biological Scientist

Anatomic Pathology

UF Veterinary Medical Center

(352) 392-2235 Ext. 4517


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