[Histonet] effect on IHC staining with the use of Cytolyt for cellblocks

[Tony Henwood]

Remember the cytolyte solution is an alcohol-based fixation. Very little
formalin, so enzyme pre-treatments will tend to chew up the sections.
Try the IPXs leaving out any enzyme unmasking. Some antibodies may not
recognise their antigens (eg p53)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
kold <@t> neo.rr.com
Sent: Saturday, 21 June 2008 1:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] effect on IHC staining with the use of Cytolyt for
cellblocks


Has anybody had any problems with IHC staining on cell block slides that
were processed with Cytolyt solution

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************