[Histonet] uneven HE staining
JCollins <@t> palmbeachpath.com
Mon Jun 16 11:39:01 CDT 2008
The way it is described sounds like thick and thin sections, a cutting problem, not a staining problem.
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Wilder
Sent: Monday, June 16, 2008 12:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] uneven HE staining
A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer. This has been an ongoing problem. She tells me it is only on biopsies, very intermittent. On one slide she might have four sections; three stain evenly and one does not. The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last. Several technicians are cutting so technique has been eliminated. They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next? We don't think it is the processing; dehydration? I do not have their HE programmed times.
Thanks for your help,
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