[Histonet] uneven HE staining

[Paula Wilder]

Hi everyone!
 
A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer.  This has been an ongoing problem.  She tells me it is only on biopsies, very intermittent.  On one slide she might have four sections; three stain evenly and one does not.  The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last.  Several technicians are cutting so technique has been eliminated.  They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next?  We don't think it is the processing; dehydration?  I do not have their HE programmed times.
 
Thanks for your help,
Paula Wilder
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