AW: [Histonet] Fite stain

Gudrun Lang gu.lang <@t>
Wed Jun 11 09:52:28 CDT 2008

Is it possible, that these spots could be mastcell-granula? It would turn
metachromatic red due to methylenblue. - but perhaps I'm totally wrong.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Martin,
Gesendet: Mittwoch, 11. Juni 2008 14:57
An: histonet
Betreff: [Histonet] Fite stain

Good morning everyone,  
I am having a problem with my Fite stains.  Most stain beautifully but I
have had trouble with a few cases.  Here are the doc's comments:
"case xxxx has some lipomembranous change- and this may not really be an
entirely false positive result. But there is clearly no explanation for the
stuff in xxxx that stains, including some fibrin. I've never seen this
before. It's not the usual round spots from inadequately rinsing off the
peanut oil...."
"A couple of days ago, I saw a granulomatous reaction that had tiny Fite
positive "blobbos" that looked like microbes in tiny cystic spaces within
the granulomas. I initially thought they might be real organisms but then I
did a TB stain and proved that they were nothing. Just moments ago, XXXX had
a case that was essentially identical. The Fite stain is working perfectly
for ID of microbes, but it seems to have a false positive kind of reactivity
that can be seen in small spaces within a granulomatous infiltrate. On an
occasional basis only."
Does anyone have any idea?  Our procedure is 2 changes of xylene/peanut oil,
air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid
alcohol, rinse, counterstain in methylene blue, air dry.
Thanks in advance,

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