[Histonet] Fite stain

Martin, Erin Erin.Martin <@t> ucsf.edu
Wed Jun 11 07:57:07 CDT 2008

Good morning everyone,  
I am having a problem with my Fite stains.  Most stain beautifully but I have had trouble with a few cases.  Here are the doc's comments:
"case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...."
"A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only."
Does anyone have any idea?  Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry.
Thanks in advance,

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