[Histonet] IHC for estrogen receptor beta

Andi Kappeler kappeler <@t> patho.unibe.ch
Mon Jun 9 11:41:11 CDT 2008

Hi all
has anybody been successfully using mouse-anti hu estrogen receptor beta,
clone 14C8 (GeneTex) on FFPE tissue? We have tried 8 different pretreatment
variants on 6 different samples of breast carcinoma (including normal
mammary glands) at Ig conc of 4 and 8 mg/l. Visualization with polymer/HRP
and DAB. If you were successful, would you mind sharing your protocol? If
you were successful with a different anti-estrogen receptor beta antibody, I
would be interested, too (clone designation, company)! Many thanks!
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

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