[Histonet] Staining for Hyaluronic acid

[Tony Henwood]

Try one of the colloidal iron methods. This seems to detect smaller
amounts of carboxylated mucins then the alcian blue methods. This is why
several dermatopathologists prefer it for skin biopsies.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Paul
Sent: Thursday, 5 June 2008 8:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Staining for Hyaluronic acid


I just finished the same technique 10 minutes ago!  Alcian blue for
hyaluronic acid, with and without hyaluronidase.  Hyaluronic acid is
seldom present in tissues in high concentration.  When the entity you
are staining is sparse, your staining is going to be light.  There is
really no way around that.  I use umbilical cord as a control.
Hyaluronic acid is present in this tissue in higher concentration than
in most other tissues.  You can also try to increase your staining
intensity by cutting your sections a little thicker.


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