[Histonet] Staining for Hyaluronic acid

Rema rema.oliver <@t> unsw.edu.au
Wed Jun 4 16:39:45 CDT 2008



I am trying to stain some rabbit skin to detect Hyaluronic acid but have 
found that my staining is very weak even though I have left the samples 
in 1% Alcian blue (pH 2.5) for 30 mins which is what standard procedures 
from Histo technique books say to do. I am also counterstaining with 
neutral red. Does anyone have a procedure that knows works or could 
suggest how I can increase the staining intensity?

Many thanks,

Rema Oliver PhD
Research Fellow
Surgical & Orthopaedic Research Laboratories
Level 1, Clinical Sciences Bldg
Prince of Wales Hospital

Ph: +61 2 9382 2654
Fax: +61 2 9382 2660
Mobile: 0419 605 034

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