[Histonet] MMA deplasticizing
Nancy W. Troiano
nancy.troiano <@t> yale.edu
Wed Jun 4 08:30:12 CDT 2008
I deplastify our MMA slides using Cellosolve, available from Fisher,
catalog #E181-4 - use two 30 minute room temp soaks to deplastify 5 micron
thick sections - use under fume hood as fumes are hazardous. Or,
alternatively, used two 5 minute soaks in acetone in a fume hood. We
follow up both deplastifications with a 5 minute soak in 70% ethanol, 5
minutes in 40% ethanol then at least 5 minutes in distilled water prior to
staining.
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