[Histonet] MMA deplasticizing

Nancy W. Troiano nancy.troiano <@t> yale.edu
Wed Jun 4 08:30:12 CDT 2008

I deplastify our MMA slides using Cellosolve, available from Fisher, 
catalog #E181-4 - use two 30 minute room temp soaks to deplastify 5 micron 
thick sections - use under fume hood as fumes are hazardous.  Or, 
alternatively, used two 5 minute soaks in acetone in a fume hood.  We 
follow up both deplastifications with a 5 minute soak in 70% ethanol, 5 
minutes in 40% ethanol then at least 5 minutes in distilled water prior to 

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