[Histonet] Toluidine Blue and H&E

Martin, Erin Erin.Martin <@t> ucsf.edu
Mon Jun 2 15:28:15 CDT 2008

Hi all,
My pathologist would like to do a combined toluidine blue and H&E.  Has anyone ever done this?  If so, would you mind sharing your procedure?  I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin.  
Thank you for your help!
Erin Martin

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