[Histonet] Storage of WGA-HRP for work solution (axon tracing)

[Gayle Callis]

Call Sigma.   However we do NOT predilute and store it, and it will be 
extremely difficult to store 5 ul in a tube, let alone even see that amount 
after you pipette it.  We do the dilution at the time of staining.  If 
reconstituting a dry WGA - I assume this is what you need, I would make it 1 
mg/ml for ease of use.  However, Sigma should have given you instructions on 
what to use for reconstitution in that data sheet.   One needs to be careful 
with lectins i.e. the buffer used.


Gayle M. Callis
HTL/HT/MT(ASCP)

----- Original Message ----- 
From: "tf" <tifei <@t> foxmail.com>
To: "histonet <@t> lists.utsouthwestern.ed" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, July 30, 2008 1:44 AM
Subject: [Histonet] Storage of WGA-HRP for work solution (axon tracing)


> Hi All:
>
> Consistent with my previous discussion on WGA-HRP use for anterograde 
> tracing, I have purchased 1 mg L7017 from Sigma.
> Given the tiny amount within the tube, do anyone have the experience in 
> choosing a proper dilution factor for storage?
> I think the best way is to dilute WGA-HRP into work solution, for example, 
> 1% for axon tracing, and stored in separate tube (5 ul per tube)?
> Or there are any other tricks?
>
> They do not provide this on information sheet.
> http://www.sigmaaldrich.com/sigma/product%20information%20sheet/l7017pis.pdf
>
> Thanks very much.
>
>
> 2008-07-30
>
>
>
> tf
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