[Histonet] Protocol for polyester wax embedding using Methacarn-fixed tissue

[shazana hilda]

Dear Histonetters,
 
I'll be processing tissue manually from Methacarn-fixed tissue using polyester wax. As a first-timer i'm not familiar with the protocol using polyester wax. They're few journals mentioned variety of protocols. So if anyone who kinds to share their protocol and experienced are greatly appreciated. For your information also, the Methacarn fixative mixture is (methanol:Chloroform:Glacial Acetic Acid,60:30:10) while the polyester wax is from Electron Microscopy Sciences deviced by Dr. H. F. Steedman. This tissue block will further use for Laser microdissection technique. Waiting for your quick response ASAP.
 
Thank You in advance.
 
Shazana Hilda Shamsuddin
MSc.of Molecular Pathology,
Dept. of Pathology,
School of Medical Sciences,
Universiti Sains Malaysia,
16150 Kubang Kerian,
Kelantan, Malaysia.
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com