[Histonet] Sodium barbital buffer substitute
Tony Henwood
AnthonyH <@t> chw.edu.au
Mon Jul 28 18:39:07 CDT 2008
Use an acetate buffer. It works well for ATPases and acid phosphatases. DO NOT use for esterases (use phosphate)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barbara Albert
Sent: Tuesday, 29 July 2008 9:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sodium barbital buffer substitute
Hi all,
I've been asked to post this question to the list. What do you use as a substitute for Sodium barbitol buffer in your muscle histochemistries?
Thanks,
Barbara Albert
UCSF Medical Center
San Francisco
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