[Histonet] vibratome questions

[Judith L. Williams]

Hi- I have a procedure for embedding mouse embryos and whole hearts in acrylamide for vibratome sectioning. If you are interested, I can send it over to you under separate email.
Judy Williams
Comparative Medicine
U of WAshington

On Wed, 23 Jul 2008, Teisha Robertson wrote:

> Good Morning All,
>  
> We are getting a vibratome in our lab and I was wondering if anyone has a protocol for embedding fresh tissue of the mouse brain?  I heard that you can embed the tissue in agrase, if so what percentage and is that all I need?  Also, will a still be able to fix my tissue in formalin?  Any suggestions would greatly be appreciated.
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Judith Williams, PhD, HT(ASCP)
Research Scientist
Department of Comparative Medicine
University of Washington
Seattle, WA 98195