[Histonet] NBT/BCIP staining for Alkaline Phosphatase

[eplurbus <@t> u.washington.edu]

Hello all,

I am condicting a bone histomorphometry study that involves staining for detection of alkaline phosphatase on methymethacrylate embedded sections. I am able to get OK results with Roche brand NBT/BCIP ready-to-use tablets but I was wondering if anyone out there would recommend another specific brand of tablets that they have had good success with.

Also, I am currently using an old bottle of Zymed Clearmount to mount the cover slips. I am not so pleased with the results. Can anyone recommend a good water based mountant?

Thanks

Alexander Dowell
University of Washington
eplurbus <@t> u.washington.edu