[Histonet] Double immunostaining with two same source 1st Antibody

Patsy Ruegg pruegg <@t> ihctech.net
Sat Jul 12 13:07:48 CDT 2008


You can still have issues with the second red detection trying to stick to
the first AB unless you do some very aggressive blocking.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of tf
Sent: Saturday, July 12, 2008 9:58 AM
To: histonet
Subject: [Histonet] Double immunostaining with two same source 1st Antibody

Hi all

Just wonder any one of you tried to perform IHC using two primary antibodies
from same source, mice, for example?

The process is that 
Mice anti-A (1st IHC)->secondary antibody labeled with GREEN
fluroscence->wash
-> Mice anti-B (2nd IHC)-> Red fluroscence

then there's no cross reaction.

thx.

2008-07-12 



tf 
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