[Histonet] Double immunostaining with two same source 1st Antibody

[tf]

Hi all

Just wonder any one of you tried to perform IHC using two primary antibodies from same source, mice, for example?

The process is that 
Mice anti-A (1st IHC)->secondary antibody labeled with GREEN fluroscence->wash
-> Mice anti-B (2nd IHC)-> Red fluroscence

then there's no cross reaction.

thx.

2008-07-12 



tf