[Histonet] Antibody advice.

[Andrea Hooper]

F4/80 from Serotec works very well in FFPE sections, haven't tried in frozen
CD4 from BD Pharmingen is beautiful in frozens, I understand it 
doesn't work in FFPE
CD3 from BD Pharmingen is beautiful in frozens, I understand it DOES 
work in FFPE
Ly-6/Gr-1 from BD Pharmingen is beautiful in frozens, I haven't tested in FFPE

>            Co-worker wants to look at macrophage using F4/80, neutrophils
>  using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable
>  source of the antibodies?
>
>  Ian.
>
>
>
>
>
>  Dr. Ian Montgomery,
>
>  Histotechnology,
>
>  I.B.L.S. Support Unit,
>
>  Thomson Building,
>
>  University of Glasgow,
>
>  Glasgow,

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