[Histonet] RE: Vector Labs Nova HRP--Red Substrate: Not Really EtOH compatible?

[C.M. van der Loos]

Jerry,The reaction product from Vector NovaRed gradually dissolves in water. Are you sure your slides didn't stay in water too long? Are your slides completely dehydrated before mounting?We use VNRed a lot and uses the following approach: After developing the red color, wash with distilled water for 2 min. Dry slides at 50C hot plate. Coverslip with VectaMount and leave slides at the hot plate for 1 hour. This preserves the red reaction product very well. If you immerse the slides in alcohols, the red color shifts a bit towards brown. Especially with double staining we don't want that.Hope this helps a bit.ChrisChris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands Date: Mon, 7 Jul 2008 10:54:49 -0700
From: JR R <rosenfeldtek <@t> hotmail.com>
Subject: [Histonet] Vector Labs Nova HRP--Red Substrate: Not Really
EtOH compatible?
To: histonet <@t> lists.utsouthwestern.edu

The Product information says: 

"NovaRED� substrate may be
dehydrated in ethanol and cleared in organic solvents such as xylene
and permanently mounted in a non-aqueous mounting medium such as
VectaMount"


But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished.

This is getting to be expensive!

Has anyone else ever had this problem with Nova Red?  Any ideas?

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology