[Histonet] Antibody advice.

[Patti Loykasek]

Ian, Is this for formalin fixed, paraffin embedded human tissue?  I am not
familiar with clone F4/80 for macrophages, but we recently added CD163 mouse
clone 10D6  that works well for monos/macs (better than CD68). For CD3 I
like mouse clone PS1, and for CD4  mouse clone 4B12 works best for us. CD4
can be a bit tricky out of paraffin, but this clone works well.  Hope this
helps. 


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA







>           Co-worker wants to look at macrophage using F4/80, neutrophils
> using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable
> source of the antibodies?
> 
> Ian. 
> 
> 
> 
> 
> 
> Dr. Ian Montgomery,
> 
> Histotechnology,
> 
> I.B.L.S. Support Unit,
> 
> Thomson Building,
> 
> University of Glasgow,
> 
> Glasgow,
> 
> G12 8QQ.
> 
> 
> 
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