[Histonet] Antibody advice.
Patti Loykasek
ploykasek <@t> phenopath.com
Tue Jul 8 09:37:34 CDT 2008
Ian, Is this for formalin fixed, paraffin embedded human tissue? I am not
familiar with clone F4/80 for macrophages, but we recently added CD163 mouse
clone 10D6 that works well for monos/macs (better than CD68). For CD3 I
like mouse clone PS1, and for CD4 mouse clone 4B12 works best for us. CD4
can be a bit tricky out of paraffin, but this clone works well. Hope this
helps.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
> Co-worker wants to look at macrophage using F4/80, neutrophils
> using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable
> source of the antibodies?
>
> Ian.
>
>
>
>
>
> Dr. Ian Montgomery,
>
> Histotechnology,
>
> I.B.L.S. Support Unit,
>
> Thomson Building,
>
> University of Glasgow,
>
> Glasgow,
>
> G12 8QQ.
>
>
>
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