[Histonet] Antibody advice.

[Liz Chlipala]

Ian

I'm assuming that you want to stain mouse tissue.  This is what we use:

F4/80 from serotec cat: MCAP497 (paraffin)
Neutrophil from serotec cat: MCA771 clone clone 7/4 (paraffin)
CD3 from dako cat: A0452 (paraffin)
CD4 from BD Pharmagen cat: 550278 (only works on frozen sections) 

Hope this helps.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Tuesday, July 08, 2008 8:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antibody advice.

            Co-worker wants to look at macrophage using F4/80,
neutrophils using Ly-6 and T-cells with CD3 and CD4. Any suggestions for
a reliable source of the antibodies?  

Ian. 

 

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

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