[Histonet] troubleshooting

[Karla Arrington]

2 questions for you all. What causes tissue sections after H&E stained fixed in formlain, to have nuclear bubbling? Is it under fixation, prolonged fixation? Also what is the best way to store for a short time, fresh, unfixed tissue either in a saline moistened gauze and refrigerated or just placing it in physiological saline at room temperature.  I am troubleshooting some problems. 
Karla Arrington
freckles9660 <@t> yahoo.com