[Histonet] Quantification for Sirius red staining

[Andrea Grantham]

I haven't done this but I have done the Picrosirius stain for people who have.
What I do is this - Don't use a counterstain.
Our imaging expert says to use a grayscale camera.
I just googled quantification of picrosirius red 
and got several hits - you might want to try this 
and see if any will give you a protocol.

Andi



At 05:14 AM 7/1/2008, Rene J Buesa wrote:
>Amy:
>Regardless of somebody or nobody doing that, it 
>is a good idea and should be done.
>The only thing is that you have to have a 
>reference point that should be a KNOWN 
>concentration reacted with the stain to refer to 
>your determinations. Ideally you should have a 
>low and high concentrations and determine a 
>correlation, in the same way as with a 
>colorimetric determination of the concentration 
>of some substance with a colored reaction.
>René J.
>
>--- On Mon, 6/30/08, Amy Lee <amylee779 <@t> yahoo.com> wrote:
>
>From: Amy Lee <amylee779 <@t> yahoo.com>
>Subject: [Histonet] Quantification for Sirius red staining
>To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
>Date: Monday, June 30, 2008, 7:30 PM
>
>Hello histonetters,
>
>I've asked a few questions here and got many big help. I appreciated all
>help you guys provided.
>
>I have another question. I've done sirius red stain on paraffin sections
>and need quantify it. I have image pro plus software. So I am thinking use it
>to measure sirius red density. I am trying to find reference that other people
>done that before (to support my idea).  Have you or anybody you know done
>that before? I googled but not many helpful info availble.
>
>Thanks for your help!
>
>Amy
>
>
>
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: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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