[Histonet] fluorescent staining on xenograft tissues

Gayle Callis gayle.callis <@t> bresnan.net
Thu Jan 31 12:03:14 CST 2008

You may be able to use the DAKO ARK kit for this.  There is an excellent 
publication in Journal of Histochemistry and Cytochemistry by Dr. Chris van 
der Loos, on the use od ARK with monoclonals on human tissues.  Hopefully, 
Chris is looking in and can comment on this.

Gayle M. Callis
Bozeman MT 59715
----- Original Message ----- 
From: "FU,DONGTAO" <fudo <@t> ufl.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 31, 2008 6:37 AM
Subject: [Histonet] fluorescent staining on xenograft tissues

> Hi,all
>  I will start fluorescent staining on xenograft tissue(human cells inject 
> into Scid mice) using Ms monoclonal antibody against human origin(like CD 
> antibodies). So there raise a problem how to reduce background on 
> xenografts when you use mouse monoclonal antibody? Does anyone have any 
> experience or any protocol I can share?
>  Thank you,
> Ann Dongtao Fu MD, Ph.D
> Lab Manager
> Dept. of Pathology
> Lab phone: 352-273-7752
> Lab FAX: 352-273-7755
> Lab address: D11-50
> PO Box: 100275
> 1600 SW Archer Road
> University of Flodrida
> Gainesville, FL 32610
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