[Histonet] fluorescent staining on xenograft tissues

[FU,DONGTAO]

Hi,all

  I will start fluorescent staining on xenograft tissue(human 
cells inject into Scid mice) using Ms monoclonal antibody against 
human origin(like CD antibodies). So there raise a problem how to 
reduce background on xenografts when you use mouse monoclonal 
antibody? Does anyone have any experience or any protocol I can 
share?

  Thank you,

Ann Dongtao Fu MD, Ph.D
Lab Manager
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610