[Histonet] Old tissue for doublecortin staining

[Anand D]

Dear Friends,
I need your help for the following situation. In my new lab, they have mouse
brain tissues stored in PBS (4 degree c) for the past one year. Now my task
is to do doublecortin immunostaining in these tissues. I tried several time
(without antigen retrieval method) but I could not able to get any staining.
However, when I tried with GFAP I could able to see some staining. Can
anybody help suggest any methods by which I can get doublecortin staining in
these old tissues?
I appreciate your help in advance,
Thank you
Anandh