[Histonet] Re: Histonet Digest, Vol 50, Issue 39
Kathy.Bucknell <@t> leica-microsystems.com
Kathy.Bucknell <@t> leica-microsystems.com
Mon Jan 28 12:13:25 CST 2008
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----- Original Message -----
From: histonet-request
Sent: 01/28/2008 06:01 PM GMT
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 50, Issue 39
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Today's Topics:
1. Cannabinoid receptor Abs (Carl Hobbs)
2. Kristensen's Sodium Formate/Formic acid recipe (jstaruk)
3. RE: Kristensen's Sodium Formate/Formic acid recipe
(Rittman, Barry R)
4. advice about microtomes please! (Patty Dunlop)
5. Motorized vs not RE: [Histonet] advice about microtomes
please! (Cheryl)
6. Re: HER2 fixation time (C.M. van der Loos)
7. thanks (louise renton)
8. FW: Lab science gets a new face on Facebook! (Luis Chiriboga)
9. Epstein Barr Virus Ab and Control Tissue (Geraci-Erck, Maria)
10. Camera Lucida (Dr. Catherine L. Ryan)
11. Re: Camera Lucida (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Sun, 27 Jan 2008 18:14:45 -0000
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] Cannabinoid receptor Abs
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001f01c86110$7f76eb10$4001a8c0 <@t> carlba65530bda>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Anyone out there use Abs against CB1/2 on frozen/pwax tissues/cells?
I would be most grateful for your views.
Thanks.
Carlos
------------------------------
Message: 2
Date: Sun, 27 Jan 2008 19:04:56 -0500
From: "jstaruk" <jstaruk <@t> masshistology.com>
Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0902019EEC4E470892B0FFC176FD6845 <@t> JimPC>
Content-Type: text/plain; charset="US-ASCII"
Does anyone have the recipe and procedure for Kristensen's Sodium
Formate/Formic acid for demineralization of teeth?
Thank you
Jim
_____________________
Jim Staruk
Mass Histology Service
www.masshistology.com
------------------------------
Message: 3
Date: Sun, 27 Jan 2008 20:28:58 -0600
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] Kristensen's Sodium Formate/Formic acid recipe
To: "jstaruk" <jstaruk <@t> masshistology.com>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EA1FDD2A141B7448B4B1AFFFCAC08DE40902FA44 <@t> UTHEVS1.mail.uthouston.edu>
Content-Type: text/plain; charset="iso-8859-1"
340 gms dried sodium formate
1700 ml. w/v 90% formic acid
Distilled water to 10 liters.
Fix as usual
Rinse in running tap water after formalin fixation.
Demineralize using agitation such as electronic stir bar.
(Difficult to over deminerlize with this formula).
Usually takes 3 to 5 times longer than 5% nitric acid.
Wash well in running tap water .
Sorry don't have a reference as I am at home but have used this for several years and prefer this to all other demineralization methods except EDTA. Worked well with many IHC techniques.
Barry
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of jstaruk
Sent: Sun 1/27/2008 6:04 PM
To: 'Histonet'
Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe
Does anyone have the recipe and procedure for Kristensen's Sodium
Formate/Formic acid for demineralization of teeth?
Thank you
Jim
_____________________
Jim Staruk
Mass Histology Service
www.masshistology.com
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------------------------------
Message: 4
Date: Sun, 27 Jan 2008 18:32:39 -0800
From: "Patty Dunlop" <pdunlop720 <@t> gmail.com>
Subject: [Histonet] advice about microtomes please!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<80ab7bc60801271832w4113190l143854a9badf532d <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello again,
I appreciate all responses about microtome of choice. I would like to ask
for guidance on my specific situation. In my facility, I will be sectioning
approximately 25-30 blocks/day of GI (upper and lower) tissue (mostly
biopsies and some polyps) and possibly prostate. I have never used a manual
microtome and do not know how repetitive it can be. In terms of ergonomics
with worries of repetitive motion and carpel tunnel, should I try to
convince my boss to get a motorized microtome, or will a manual suffice? I
know that motorized microtomes are probably twice as expensive, and although
I would feel more comfortable using the same one that I am used to, maybe it
would be "going overboard" to get the motorized in my situation.
Advice please!
Patty
------------------------------
Message: 5
Date: Sun, 27 Jan 2008 21:20:51 -0600
From: "Cheryl" <tkngflght <@t> yahoo.com>
Subject: Motorized vs not RE: [Histonet] advice about microtomes
please!
To: "'Patty Dunlop'" <pdunlop720 <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00da01c8615c$c9a728c0$6701a8c0 <@t> FSROGER>
Content-Type: text/plain; charset="us-ascii"
Hi Patti--
While I agree if you can afford one, get a motorized, how much is that
carpal surgery and the associated problems vs. the price of a microtome? It
isn't the end of the world if you don't get it, though. I've been a tech
for 25 years and haven't had this resource available at most of my jobs. In
cutting, I can rock n roll over 300 biopsy blocks with levels in a day (yes,
good sections but only cutting--not getting up to do anything else). I
haven't worked 100% on a histo bench this whole time but in the days of
cutting like this every day--it was my SHOULDERS that got tired, not my
wrists. If you're prone to carpal and a histotech--you're probably going to
get it eventually. But you can slow it down and minimize the risk. (That
department is called risk 'management', not risk elimination!!)
The bigger issue is sitting high enough & close enough that you aren't
holding your shoulders up with your neck muscles and that you alternate
activities, stretch, take breaks...etc. The best microtome for your body
might be the worst for someone else. Try to get a demo model and TRY IT.
Pay attention to any aches or pains because although your body will adjust
and get used to the situation--that doesn't mean the damage has stopped
occuring. If it doesn't fit you--try the next model!
PREVENTION is ALWAYS a better investment than banking on a reasonable cure.
Taking care of your body is a big part. Make sure you take enough B vitamins
(soft tissue support) drink enough water to support your own system (muscles
and connective tissue) and any other supplements a good qualified
nutrition/health food consultant might suggest for you. It is amazing how
many of us don't drink water because we have to leave the lab to do so!
Your state deparment of labor will have an ergonomics division and can send
someone out to evalutate your setup to be sure you are maximizing the
reduction of risk for repetitive motion and seat position for good health.
One of my labs sent me through ergo training (Washington State) and I still
use the manuals for setting up workstations in my office and when I travel
and work in temp situations. I type 6-8 hours a day at a computer and can
be on the phone over 40 hrs a week--same issues--and I'm still going strong
(knock on wood!)
Best wishes in your search!
Cheryl
Cheryl R. Kerry, HT(ASCP), BA
Full Staff Inc.
Staffing the lab - One GREAT tech at a time.
281.852.9457 office
281.883.7704 cell
800.756.3309 fax and alternate phone
admin <@t> fullstaff.org
www.fullstaff.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patty Dunlop
Sent: Sunday, January 27, 2008 8:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] advice about microtomes please!
Hello again,
I appreciate all responses about microtome of choice. I would like to ask
for guidance on my specific situation. In my facility, I will be sectioning
approximately 25-30 blocks/day of GI (upper and lower) tissue (mostly
biopsies and some polyps) and possibly prostate. I have never used a manual
microtome and do not know how repetitive it can be. In terms of ergonomics
with worries of repetitive motion and carpel tunnel, should I try to
convince my boss to get a motorized microtome, or will a manual suffice? I
know that motorized microtomes are probably twice as expensive, and although
I would feel more comfortable using the same one that I am used to, maybe it
would be "going overboard" to get the motorized in my situation.
Advice please!
Patty
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 28 Jan 2008 09:13:47 +0100
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] Re: HER2 fixation time
To: histonet <@t> lists.utsouthwestern.edu
Cc: Keith.Danielson <@t> uphs.upenn.edu
Message-ID: <29bafb295d7c.295d7c29bafb <@t> amc.uva.nl>
Content-Type: text/plain; charset=us-ascii
Hi,
During my workshop in Denver 'how to make a new antibody work for IHC?'
I showed the audience a picture taken from the paper of Shi, Liu and
Taylor in J Histochem Cytochem 55:105-109, 2007. In this paper there is
the result shown of a fixation experiment from 6 hs to 30 days.
According to the images presented there is no loss of Her2 within that
time frame.
Cheers,
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Fri, 25 Jan 2008 12:15:56 -0500
From: "Danielson, Keith" <Keith.Danielson <@t> uphs.upenn.edu>
Subject: RE: [Histonet] Re: HER2 fixation time
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Della Speranza, Vinnie"
<dellav <@t> musc.edu>, "Dawson, Glen"
<GDawson <@t> dynacaremilwaukee.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Hello,
The link below might be of interest. The article cites an interesting
study by Arber in 2002 (Arber DA. Effect of prolonged formalin fixation
on the immunohistochemical reactivity of breast markers. Appl
Immunohistochem Mol Morphol. 2002;10:183-186.). He demonstrated that
prolonged fixation of breast tissues in formalin for 7-14 days did not
significantly affect immunoreactivity of Her2. I have not been able to
get the full article yet -- I would appreciate receiving a PDF of it.
http://findarticles.com/p/articles/mi_qa3725/is_200710/ai_n21099762
I am currently growing Her2 expressing cells in cell culture and plan
to examine the effect of formalin fixation time and paraffin embedding
on immnunoreactivity by IHC. Basically, I am making some control Her2
paraffin blocks for validation purposes.
Keith Danielson, PhD
Department of Pathology
Pennsylvania Hospital
Philadelphia, PA 19107
------------------------------
Message: 7
Date: Mon, 28 Jan 2008 10:49:15 +0200
From: "louise renton" <louise.renton <@t> gmail.com>
Subject: [Histonet] thanks
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<e483362e0801280049y3d925012l7e02d0bfc90f1ada <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Thanks to all who responded to my question re charging for immunos,
much appreciated. haev a good week everyboby!
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 8
Date: Mon, 28 Jan 2008 07:59:15 -0500
From: "Luis Chiriboga" <Luis.Chiriboga <@t> med.nyu.edu>
Subject: [Histonet] FW: Lab science gets a new face on Facebook!
To: "IHCRG" <ihcrg <@t> googlegroups.com>, "HISTONET"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <KFEIIJOCLBABEKFDAEHPOEDMHCAA.Luis.Chiriboga <@t> med.nyu.edu>
Content-Type: text/plain; charset=us-ascii
-----Original Message-----
From: owner-mls-l <@t> ms3.hunter.cuny.edu
[mailto:owner-mls-l <@t> ms3.hunter.cuny.edu]On Behalf Of Regina Linder
Sent: Friday, January 25, 2008 3:25 PM
To: mls-l <@t> hunter.cuny.edu
Subject: Fwd: Lab science gets a new face on Facebook!
Hello Everyone: As the semester begins, check out this terrific site.
"Labs are Vital" is on Facebook, with info on careers in all kinds of labs,
scholarship contests, etc. You can download terrific posters to raise
consciousness in your hospitals and your kid's schools. All the best, Dr. L
If you are unable to see the email clearly, please follow the link below:
[ please click here]
Labs Are Vital officially joined the Facebook generation in December. In
an effort to draw future professionals to clinical lab science, Labs Are
Vital has launched a new recruitment campaign on Facebook with information
on educational and career opportunities, and a $2,500 scholarship contest.
An announcement letter about the Facebook campaign and scholarship
opportunities was also mailed to over 10,000 high school counselors and
science department heads.
Contest entrants may submit a video, advertisement or T-shirt design that
encourages others to consider careers in laboratory medicine. Semifinalist
submissions will be posted online, where Facebook members will vote on those
that most creatively illustrate why clinical lab science is cutting edge and
how laboratorians make a difference.
Abbott is joined in this effort by ASCLS, APHL and Blood Systems, Inc.
Equipment donation
call for entries!
Labs Are Vital invites all accredited CLS/CLTS programs to apply for our
latest round of equipment donations. So far, 43 programs have been selected
to receive state-of-the-art diagnostic equipment, supplies and service
through the Labs Are Vital $1 million donation program. You'll find winner
profiles, and have the opportunity to apply for the next round of donations.
But don't wait-applications are due March 31, 2008! For application, click
here.
The polls have closed and
the results are in!
Thanks so much to the many of you who participated in our recent Labs Are
Vital survey. The response rate was gratifying. While we are still in the
process of analyzing the data, the top-line response lets us know that the
majority of you have been instrumental in passing the word about Labs Are
Vital, which has resulted in many new members. And your suggestions about
what you would like us to add will only help improve Labs Are Vital and make
it even better in 2008.
We'll be holding our drawing for 100 Labs Are Vital lab coats in
mid-February, and will announce the winners shortly thereafter. We'll also
be sharing more of the survey results with you-the lab professionals who
keep health care moving forward.
Send a copy of this e-mail to a colleague Unsubscribe
If you are unable to see this email clearly, please click here
Download
Labs Are Vital posters
Our Labs Are Vital posters not only celebrate the remarkable contributions
of lab professionals, they've also proven to be quite popular with
laboratorians and the general public alike. To download one or more of this
popular series, click here.
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Message: 9
Date: Mon, 28 Jan 2008 08:02:39 -0500
From: "Geraci-Erck, Maria" <maria.geraci-erck <@t> spcorp.com>
Subject: [Histonet] Epstein Barr Virus Ab and Control Tissue
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B86C0D639BBC3042ABCD2A31651489C502A81428 <@t> kenmsg40.us.schp.com>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
I need to label monkey tissue for Epstein Barr Virus. Does anyone out
there have experience with this procedure? I would like to know if
there is a particular Ab that would identify an active infection. There
are a number of Ab's out there and I was wondering if there is a
favorite that gives consistent results. I am also in need of positive
control tissue. I have e-mailed Pantomics and unfortunately, they did
not have a block. I found a company that sells slides, but in my
opinion, they are pricey. Any guidance would be appreciated.
Maria
Maria Geraci-Erck
Schering-Plough Research Institute
Special Techniques Laboratory
556 Morris Avenue, Bldg. S-12
Summit, New Jersey 07901-1002
Phone: (908) 473-4284
Fax: (908) 473-4420
maria.geraci-erck <@t> spcorp.com
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Message: 10
Date: Mon, 28 Jan 2008 13:25:31 -0400
From: "Dr. Catherine L. Ryan" <ryan <@t> upei.ca>
Subject: [Histonet] Camera Lucida
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <479DD7C1.12905.349CDCCF <@t> localhost>
Content-Type: text/plain; charset=US-ASCII
Hello everyone: I am interested in purchasing a camera lucida
attachment for my scope.Does anyone have one they are no longer
using and would be interested in selling?
Cathy Ryan
Dr. Catherine L. Ryan
Department of Psychology
University of Prince Edward Island
Charlottetown, P.E.I., Canada, C1A 4P3
Ph# (902)566-0323/ FAX#(902)628-4359
email: RYAN <@t> UPEI.CA
------------------------------
Message: 11
Date: Mon, 28 Jan 2008 09:36:54 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Camera Lucida
To: ryan <@t> upei.ca, histonet <@t> lists.utsouthwestern.edu
Message-ID: <27813.7341.qm <@t> web61224.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I have seen one or two in e-Bay
Ren■ J.
"Dr. Catherine L. Ryan" <ryan <@t> upei.ca> wrote:
Hello everyone: I am interested in purchasing a camera lucida
attachment for my scope.Does anyone have one they are no longer
using and would be interested in selling?
Cathy Ryan
Dr. Catherine L. Ryan
Department of Psychology
University of Prince Edward Island
Charlottetown, P.E.I., Canada, C1A 4P3
Ph# (902)566-0323/ FAX#(902)628-4359
email: RYAN <@t> UPEI.CA
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