[Histonet] CD25 - Rat

[koellingr <@t> comcast.net]

Joost,
mainly mouse but some rat work with CD25.  CD25 is the low affinity IL-2R alpha.  So found on T-cells that are activated a lot.  But as with a lot of these markers you can find increased expression in activating circumstances and find CD25 on macrophages and dendritic cells also.  But shouldn't be as much as you seem to describe. PALS I agree, less in B-cell area, marginal zone macs maybe OK.  Even red pulp lymphocytes ok since there is constitutive expression on Treg cells.  But capillaries, all over?  Something is wrong there if this is just a normal, adult rat that hasn't had something like a sham injection of some protein or immunoglobulin.  What is your detection?  If possible, let someone flow rat lymphoid tissue (same strain) and for them it would be a snap to do 2-color flow for CD3/CD25, mac marker/CD25, etc and you will get a feel of how many kinds of cells and percentages should be staining in your tissue.  Remembering back to my projects wouldn't help you since your str
ains and IHC detection are probably much different.  But if this is a normal, control rat, sounds like a bit too much staining to me.

Ray Koelling
PhenoPath Labs
Seattle, WA

-------------- Original message -------------- 
From: "Bruijntjes, J.P. (Joost)" <joost.bruijntjes <@t> tno.nl> 

> Hi all 
> 
> 
> 
> Is anyone of you familiar with staining rat lymphoid tissues with an 
> antibody directed against CD25? 
> 
> In the white pulp there is a lot of positive staining on different 
> lymphocytes in the PALS, (less in the) B-cell area. In the marginal zone 
> positive staining is seen on some large cells, most probably 
> macrophages. 
> 
> In the red pulp there is a lot of staining on different type of 
> lymphocytes too. But I see a lot of positive staining on (most probably) 
> capillaries (endothelial cells) . Not a few capillaries, but all. 
> 
> Does it sound familiar to anyone of you? 
> 
> 
> 
> Joost Bruijntjes 
> 
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