AW: [Histonet] Whole Mount Prostates
gu.lang <@t> gmx.at
Wed Jan 23 09:36:57 CST 2008
The prostatae are fixed in NBF at least for two days, then marked with black
and green ink and sliced up in 8-10 mm slides.
We work with the big cassettes and molds. The VIP-protocol for the routine
specimen was just doubled in each station.
The thick slides tend to bend up in the center. So embedding should be done
with enough power to stick them on the ground.
Our routine microtomes are sliding microtomes, so the prostatae are also cut
on these and that works fine.
The HE protocol is the same as with routine specimen.
Hope this helps.
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Metzger,
Gesendet: Dienstag, 22. Jänner 2008 21:03
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Whole Mount Prostates
Can anyone send or refer me to a processing protocol for whole mount
prostates? We haven't done them before and need some guidance. Any other
secrets or tricks on the cutting and staining would be greatly appreciated.
Ken Metzger HTL(ASCP)
500 Chipeta way
Salt Lake City, UT 84108
801.583.2787 ext 3101
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