[Histonet] Mucin containing cells too blue

Greg Dobbin gvdobbin <@t> ihis.org
Tue Jan 22 14:24:05 CST 2008

Hi Folks,
I have been using the Surgipath Selectech reagents (H&E) for almost 3
months now and I have not had a single complaint about staining...until
now.  Suddenly the mucin containing cells are too blue.  My first
thought was to make fresh Define (the Surgipath differentiator).  This
did not fix the problem.  Next I replaced the hematoxylin with fresh
(not supposed to have to change before 2500 slides, but changed at 1500
slides to see if that would correct the problem). It has not.  

I was sure that fresh hematoxylin (and thererfore correct pH) would
rectify the problem.  What are the other usual suspects here?  I should
also mention our H&E's are done on the Leica Autostainer.
I look forward to receiving your collective insight!

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

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