[Histonet] Mucin containing cells too blue

[Greg Dobbin]

Hi Folks,
I have been using the Surgipath Selectech reagents (H&E) for almost 3
months now and I have not had a single complaint about staining...until
now.  Suddenly the mucin containing cells are too blue.  My first
thought was to make fresh Define (the Surgipath differentiator).  This
did not fix the problem.  Next I replaced the hematoxylin with fresh
(not supposed to have to change before 2500 slides, but changed at 1500
slides to see if that would correct the problem). It has not.  

I was sure that fresh hematoxylin (and thererfore correct pH) would
rectify the problem.  What are the other usual suspects here?  I should
also mention our H&E's are done on the Leica Autostainer.
I look forward to receiving your collective insight!
Greg



Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

Statement of Confidentiality
This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization.  If you have received this communication in error, please notify the sender immediately.  If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system.  

D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique.